Author: Kenworthy, Rachael; Lambert, Diana; Yang, Feng; Wang, Nan; Chen, Zihong; Zhu, Haizhen; Zhu, Fanxiu; Liu, Chen; Li, Kui; Tang, Hengli
Title: Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation Document date: 2009_9_3
ID: uvf5qzfd_20
Snippet: For flow cytometry, GS5 cells were fixed 48 h after treatment in a solution of 2% paraformaldehyde and analyzed with a FACSCanto flow cytometer (BD Biosciences, San Jose, CA, USA). Mean GFP intensity was plotted, and that of the sh-NTC sample was normalized to 100%......
Document: For flow cytometry, GS5 cells were fixed 48 h after treatment in a solution of 2% paraformaldehyde and analyzed with a FACSCanto flow cytometer (BD Biosciences, San Jose, CA, USA). Mean GFP intensity was plotted, and that of the sh-NTC sample was normalized to 100%.
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