Selected article for: "chain reaction and PCR amplify"
Author: Lan, Han-hong; Wang, Cui-mei; Chen, Shuang-shuang; Zheng, Jian-ying
Title: siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris
  • Document date: 2019_10_1
    • ID: qgrhh3r4_3
    Snippet: Reverse transcription polymerase chain reaction (RT-PCR). Total RNA was extracted from 0.1 g symptomatic leaves of P. equestris with Trizol reagent (Invitrogen, Carlsbad, CA, USA) as instruction. RT-PCR was performed with the primers to amplify the coat protein (CP) gene of CymMV and ORSV with Invitrogen SuperScript IV VILOTM kit (ThermoFisher, Waltham, MA, USA) as instruction......
    Document: Reverse transcription polymerase chain reaction (RT-PCR). Total RNA was extracted from 0.1 g symptomatic leaves of P. equestris with Trizol reagent (Invitrogen, Carlsbad, CA, USA) as instruction. RT-PCR was performed with the primers to amplify the coat protein (CP) gene of CymMV and ORSV with Invitrogen SuperScript IV VILOTM kit (ThermoFisher, Waltham, MA, USA) as instruction.

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