Author: Lan, Han-hong; Wang, Cui-mei; Chen, Shuang-shuang; Zheng, Jian-ying
Title: siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris Document date: 2019_10_1
ID: qgrhh3r4_9_1
Snippet: e assembled contigs from sRNAs covered about 99% of the CymMV and ORSV ge- Fig. 3A and B) , with an average sequencing depth of 350× and 400×, respectively. RT-PCR and rapid amplification of cDNA ends were respectively used to amplify the gaps between siRNA contigs and terminal sequences using primers designed according to the sequences of Cym-MV and ORSV genomes (data not shown). The obtained PCR fragments were sequenced using the Sanger metho.....
Document: e assembled contigs from sRNAs covered about 99% of the CymMV and ORSV ge- Fig. 3A and B) , with an average sequencing depth of 350× and 400×, respectively. RT-PCR and rapid amplification of cDNA ends were respectively used to amplify the gaps between siRNA contigs and terminal sequences using primers designed according to the sequences of Cym-MV and ORSV genomes (data not shown). The obtained PCR fragments were sequenced using the Sanger method and assembled to produce the near complete genomes of both viruses. Phylogenetic analysis using deduced RdRP sequences of CymMV and ORSV confirmed their relatedness to the genus Potexvirus and the genus Tobamovirus, respectively ( Supplementary Fig. 1 ).
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