Author: Stewart, Meredith E.; Roy, Polly
Title: Structure-based identification of functional residues in the nucleoside-2'-O-methylase domain of Bluetongue virus VP4 capping enzyme Document date: 2015_2_24
ID: vzel6r43_17
Snippet: Expression of viral proteins was monitored by western immunoblot using specific polyclonal antibodies that recognize either VP3 or NS1. Whole cell lysates and fractions were resolved on SDS-PAGE gels, and transferred to a nitrocellulose membrane (0.45 lm, Amersham) by the standard semi-dry transfer protocol......
Document: Expression of viral proteins was monitored by western immunoblot using specific polyclonal antibodies that recognize either VP3 or NS1. Whole cell lysates and fractions were resolved on SDS-PAGE gels, and transferred to a nitrocellulose membrane (0.45 lm, Amersham) by the standard semi-dry transfer protocol.
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