Selected article for: "agarose gel and initial denaturation step"
Author: Galindo, I; Hernáez, B; Muñoz-Moreno, R; Cuesta-Geijo, M A; Dalmau-Mena, I; Alonso, C
Title: The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection
  • Document date: 2012_7_5
    • ID: qfm61wmx_23
    Snippet: RNA preparation and RT-PCR analysis for XBP1. Total RNA from cultured cells was isolated with TRI-Reagent (Sigma-Aldrich) following the manufacturer's instructions. Reverse transcription reactions (RT-PCR) were carried out using the SuperScript One Step RT-PCR (Invitrogen), following the manufacturer's instructions. The primers used were: forward primer: 5 0 -CCTTGTAGTTGAGAAC CAGG-3 0 and reverse primer 5 0 -GGGGCTTGGTATATATGTGG-3 0 . Amplificati.....
    Document: RNA preparation and RT-PCR analysis for XBP1. Total RNA from cultured cells was isolated with TRI-Reagent (Sigma-Aldrich) following the manufacturer's instructions. Reverse transcription reactions (RT-PCR) were carried out using the SuperScript One Step RT-PCR (Invitrogen), following the manufacturer's instructions. The primers used were: forward primer: 5 0 -CCTTGTAGTTGAGAAC CAGG-3 0 and reverse primer 5 0 -GGGGCTTGGTATATATGTGG-3 0 . Amplification conditions included an initial step of 45 1C for 30 s, followed by a denaturation step of 94 1C for 2 min, 30 cycles of 94 1C for 30 s, 55 1C for 30 s and 70 1C for 30 s. PCR products were resolved by electrophoresis in a 2% agarose gel and stained with SYBR Green.

    Search related documents:
    Co phrase search for related documents
    • agarose gel and denaturation step: 1, 2, 3, 4, 5, 6
    • agarose gel and initial step: 1, 2, 3, 4
    • agarose gel and PCR product: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • agarose gel and reverse transcription reaction: 1, 2, 3, 4
    • agarose gel and RT PCR analysis: 1, 2, 3, 4, 5, 6
    • agarose gel and SuperScript Step RT PCR Invitrogen: 1, 2
    • agarose gel and SYBR Green stain: 1
    • agarose gel and transcription reaction: 1, 2, 3, 4, 5, 6
    • amplification condition and RNA preparation: 1
    • cultured cell and reverse transcription reaction: 1, 2
    • cultured cell and RT PCR analysis: 1
    • cultured cell and RT PCR reverse transcription reaction: 1, 2
    • cultured cell and transcription reaction: 1, 2
    • denaturation step and initial step: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • denaturation step and PCR product: 1, 2, 3, 4
    • initial step and PCR product: 1
    • initial step and RNA preparation: 1, 2
    • PCR product and RT PCR reverse transcription reaction: 1, 2, 3, 4, 5, 6, 7, 8
    • PCR product and transcription reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11