Selected article for: "difficulty overcome and high throughput"

Author: Burton, Aaron S.; Di Stefano, Marco; Lehman, Niles; Orland, Henri; Micheletti, Cristian
Title: The elusive quest for RNA knots
  • Document date: 2016_2_1
  • ID: s3tdllby_15_1
    Snippet: for knotted RNAs. Previous biochemical analyses raised the possibility that two naturally-occurring RNA intron sequences were capable of adopting knotted conformations, but the presence of a physical knot could not be confirmed irrefutably with the available data. 45 A significant challenge for the unambiguous confirmation of true knots in RNA is the difficulty associated with solving RNA structures; this challenge could be overcome by the develo.....
    Document: for knotted RNAs. Previous biochemical analyses raised the possibility that two naturally-occurring RNA intron sequences were capable of adopting knotted conformations, but the presence of a physical knot could not be confirmed irrefutably with the available data. 45 A significant challenge for the unambiguous confirmation of true knots in RNA is the difficulty associated with solving RNA structures; this challenge could be overcome by the development of an appropriate high-throughput assay to detect knotted RNAs, especially when present as isoforms coexisting with other stable unknotted conformations. The lack of such systematic assays is, at present, the main obstacle toward advancing our understanding of the incidence of physical knots in RNAs.

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