Selected article for: "International license and Qiagen kit"
Author: Sneha Rath; Eliza Prangley; Jesse Donovan; Kaitlin Demarest; Yigal Meir; Ned Wingreen; Alexei Korennykh
Title: Concerted 2-5A-Mediated mRNA Decay and Transcription Reprogram Protein Synthesis in dsRNA Response
  • Document date: 2018_12_4
    • ID: ng5c7xai_49
    Snippet: qPCR RNA was purified using the RNeasy kit (Qiagen). RNA quality was assessed using BioAnalyzer NanoChip (Agilent) and extent of rRNA cleavage was quantified using . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484675 doi: bioRxiv preprint GelQuant.NET (Biochem Lab Solutions, http://biochemlabsolutions.com/). Within.....
    Document: qPCR RNA was purified using the RNeasy kit (Qiagen). RNA quality was assessed using BioAnalyzer NanoChip (Agilent) and extent of rRNA cleavage was quantified using . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484675 doi: bioRxiv preprint GelQuant.NET (Biochem Lab Solutions, http://biochemlabsolutions.com/). Within each experiment, equal amounts (ng) of RNA were converted to cDNA using oligo-dT 18 as primer and the High Capacity Reverse Transcriptase kit (Applied Biosystems). qPCR was performed using Power SYBR Green PCR Master Mix (Life Technologies).

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