Author: Hu, Yongwu; Wen, Jie; Tang, Lin; Zhang, Haijun; Zhang, Xiaowei; Li, Yan; Wang, Jing; Han, Yujun; Li, Guoqing; Shi, Jianping; Tian, Xiangjun; Jiang, Feng; Zhao, Xiaoqian; Wang, Jun; Liu, Siqi; Zeng, Changqing; Wang, Jian; Yang, Huanming
Title: The M Protein of SARS-CoV: Basic Structural and Immunological Properties Document date: 2016_11_28
ID: xzlcyn3l_48
Snippet: Synthetic oligopeptides were fixed on 96-well plates at 1 μg/mL in a volume of 100 μL for each well. 100 μL Dilution Solution BSA (3%) was added to each well and then mixed with 10 μL serum. After being incubated for 60 min at 37°C and washed three times (5 min each) with PBST (100 mM Tris, 120 mM NaCl, 0.1% Tween-20, pH 7.9), 100 μL enzymes-linked solutions for Goat anti-human IgG (Beijing Zhongshan Company) were added, incubated again for.....
Document: Synthetic oligopeptides were fixed on 96-well plates at 1 μg/mL in a volume of 100 μL for each well. 100 μL Dilution Solution BSA (3%) was added to each well and then mixed with 10 μL serum. After being incubated for 60 min at 37°C and washed three times (5 min each) with PBST (100 mM Tris, 120 mM NaCl, 0.1% Tween-20, pH 7.9), 100 μL enzymes-linked solutions for Goat anti-human IgG (Beijing Zhongshan Company) were added, incubated again for 20 min at 37°C, and then washed. The peroxidase reaction was visualized using the o-pheylenediamine solution as substrate. After 10 min incubation at 37°C, the reaction was stopped by adding 50 μL of 4 M sulphuric acid to each well. Optical density (O.D.) at 450 nm was measured with an automatic ELISA reader.
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