Title: The organization of endoplasmic reticulum export complexes Document date: 1996_10_1
ID: xxlcdbqi_14
Snippet: where Sc.p. is the mean surface of the cell projection (determined by the phase-contrast light microscopy), and Hc. is the mean height of the cell (determined by TEM). For estimation of the mean cell volume using the second method, NRK and RBL cells were grown on 35-mm tissue-culture dishes (Costar Corp., Cambridge, MA), fixed in the dish in 2.5% GA in PBS for 1 h at rt, and processed for TEM as described above. The cell pellet was cut with a dia.....
Document: where Sc.p. is the mean surface of the cell projection (determined by the phase-contrast light microscopy), and Hc. is the mean height of the cell (determined by TEM). For estimation of the mean cell volume using the second method, NRK and RBL cells were grown on 35-mm tissue-culture dishes (Costar Corp., Cambridge, MA), fixed in the dish in 2.5% GA in PBS for 1 h at rt, and processed for TEM as described above. The cell pellet was cut with a diamond knife on a Reichert ultramicrotome 2E. 60-65-nm sections were counterstained as described above. Approximately 1,200 RBL and 800 NRK cells were randomly chosen and photoenlarged to a magnification of 14,000. The mean surface of the cells on sections were measured by the point-counting method (Weibel, 1979) . The mean cell volume was found according to formula (Weibel and Gomez, 1962) :
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