Selected article for: "goat serum and normal goat serum"
Author: Teoh, Kim-Tat; Siu, Yu-Lam; Chan, Wing-Lim; Schlüter, Marc A.; Liu, Chia-Jen; Peiris, J. S. Malik; Bruzzone, Roberto; Margolis, Benjamin; Nal, Béatrice
Title: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis
  • Document date: 2010_11_15
    • ID: ufw13pjx_35
    Snippet: BIOCHIP slides containing SARS-CoV–infected and uninfected Vero E6 cells (Euroimmun, Lübeck, Germany) were used according to the manufacturer's instructions. Cells were permeabilized with 0.2% Tween-20 in 1X PBS and then blocked with 10% normal goat serum (NGS, heat-inactivated, Zymed, Invitrogen) in 0.2% Tween-20, 1X PBS. Primary and secondary antibodies conjugated to fluorescein isothiocyanate (FITC) and Texas Red (TxRed) were prepared in 5%.....
    Document: BIOCHIP slides containing SARS-CoV–infected and uninfected Vero E6 cells (Euroimmun, Lübeck, Germany) were used according to the manufacturer's instructions. Cells were permeabilized with 0.2% Tween-20 in 1X PBS and then blocked with 10% normal goat serum (NGS, heat-inactivated, Zymed, Invitrogen) in 0.2% Tween-20, 1X PBS. Primary and secondary antibodies conjugated to fluorescein isothiocyanate (FITC) and Texas Red (TxRed) were prepared in 5% NGS in 0.2% Tween-20, 1X PBS. Cells were incubated at room temperature for 30 min with primary antibodies, washed three times, followed by incubation with secondary antibodies for an additional 30-min period. For Vero E6 cells transfected with pEYFP-PALS1 and pcDNA-HA-E (wt), cells were fixed with 4% PFA in 1X PBS for 15 min and subsequently immunostained as described above.

    Search related documents:
    Co phrase search for related documents
    • 1x PBS and fluorescein isothiocyanate: 1
    • 1x PBS and goat serum: 1
    • 1x PBS and primary antibody: 1, 2
    • 1x PBS and room temperature: 1, 2, 3, 4, 5
    • FITC fluorescein isothiocyanate and fluorescein isothiocyanate: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • FITC fluorescein isothiocyanate and primary antibody: 1, 2, 3
    • FITC fluorescein isothiocyanate and room temperature: 1, 2, 3, 4, 5
    • fluorescein isothiocyanate and primary antibody: 1, 2, 3
    • fluorescein isothiocyanate and room temperature: 1, 2, 3, 4, 5, 6, 7
    • goat serum and primary antibody: 1, 2, 3, 4, 5, 6, 7, 8
    • goat serum and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • heat inactivate and room temperature: 1, 2
    • manufacturer instruction and room temperature: 1
    • primary antibody and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25