Selected article for: "fusion protein and p62 region"
Title: The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation
  • Document date: 1990_9_1
    • ID: rmjv56ia_15
    Snippet: Construction of the Fusion Protein-coding Plasmids pC62alphaG and pC62dhfr. Plasmid pGEMI-SFV (also called pG-SFV-15/5; Melancon and Garoff, 1986 ) contains a re, engineered eDNA copy of the SFV 26S mRNA sequences cloned as a Barn HI fragment in the Barn HI site of the polylinker downstream of the SP6 promoter in the plasmid pGEM1 (Promaga Biotech). From the SFV plasmid, a 2,381 hp-long Pvu I-Xho I fragment, containing the coding sequences for th.....
    Document: Construction of the Fusion Protein-coding Plasmids pC62alphaG and pC62dhfr. Plasmid pGEMI-SFV (also called pG-SFV-15/5; Melancon and Garoff, 1986 ) contains a re, engineered eDNA copy of the SFV 26S mRNA sequences cloned as a Barn HI fragment in the Barn HI site of the polylinker downstream of the SP6 promoter in the plasmid pGEM1 (Promaga Biotech). From the SFV plasmid, a 2,381 hp-long Pvu I-Xho I fragment, containing the coding sequences for the capsid protein and the NH2-terminai region of the p62 protein, was isolated. The Xho I-Pvu I fragments from pGEMI-SFV, pGEM2alphaGX and pGEM2dhfrX were isolated and ligated at a 1:1 molar ratio to obtain pC62alphaG and pC62dhfr, respectively. Plasmid DNAs from ampicillin-resistant colonies were screened and compared to the starting vectors by restriction analysis.

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