The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation

We conclude that the 40 residue peptide from the p62 NH2-terminal region confers a translocation positive phenotype to the p62-globin and p62-dhfr polypeptides and therefore must contain a functional signal sequence. The translocated fusion proteins were also shown to be glycosylated. This must involve Asn~3 of the p62 peptide as it is part of the only potential glycosylation site on the hybrid polypeptides (Garoff et al., 1980 ; references on dhfr sequence in legend to Fig. 1) , Finally, we can also conclude that the p62 signal sequence does not provide a stable membrane anchor to the translocated chain.