Title: The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope Document date: 1992_12_2
ID: vznqgnzd_28
Snippet: the pore membrane, we carried out double immunofluorescence with mAb 414 (Fig. 2 B) . This mAb reacts with several nucleoporins (7) (all proteins of the NPC, excluding integral proteins of the pore membrane). A comparison of the two staining patterns (Fig. 2, A and B ) showed considerable congruity, not only between the densely punctate pattern at the nuclear surface, but also between the more dispersed punctate pattern observed in the cytoplasm......
Document: the pore membrane, we carried out double immunofluorescence with mAb 414 (Fig. 2 B) . This mAb reacts with several nucleoporins (7) (all proteins of the NPC, excluding integral proteins of the pore membrane). A comparison of the two staining patterns (Fig. 2, A and B ) showed considerable congruity, not only between the densely punctate pattern at the nuclear surface, but also between the more dispersed punctate pattern observed in the cytoplasm. The matching patterns in the cytoplasm suggest that gp210:HA and at least some of the nucleoporins are present as assembled structures in the ER. These structures might be similar to annulate lamellae (15) . Together these data suggest that epitope tagging of gp210 does not interfere with sorting.
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