Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_12
Snippet: A. tumefaciens (strain LBA4404) cultures carrying plant expression vectors were grown overnight in LB medium supplemented with appropriate antibiotics, collected by centrifugation, resuspended in agroinfiltration buffer (10 mM MES, pH 5.7, 10 mM MgCl 2 and 150 mM acetosyringone) to an OD 600 of 0.5 and incubated for 2 h at room temperature. Middle leaves of six-week old N. benthamiana plants were infiltrated using a syringe without a needle......
Document: A. tumefaciens (strain LBA4404) cultures carrying plant expression vectors were grown overnight in LB medium supplemented with appropriate antibiotics, collected by centrifugation, resuspended in agroinfiltration buffer (10 mM MES, pH 5.7, 10 mM MgCl 2 and 150 mM acetosyringone) to an OD 600 of 0.5 and incubated for 2 h at room temperature. Middle leaves of six-week old N. benthamiana plants were infiltrated using a syringe without a needle.
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