Selected article for: "ph sodium citrate buffer and sodium citrate buffer"
Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies
  • Document date: 2019_2_12
    • ID: y47ahl3p_18
    Snippet: Clarified leaf extracts were subjected to IMAC (scFv-TM43-E10) and Protein A (scFv-Fc-TM43-E10) purification. The scFv-TM43-E10 antibodies were eluted with extraction buffer containing 250 mM imidazol. The scFv-Fc-TM43-E10 protein was eluted with 100 mM sodium citrate buffer, pH 3.0. Purified scFv-TM43-E10 and scFv-Fc-TM43-E10 antibody fragments were further analysed by sizeexclusion chromatography (SEC) on Superdex 200-16/60 column (GE Healthcar.....
    Document: Clarified leaf extracts were subjected to IMAC (scFv-TM43-E10) and Protein A (scFv-Fc-TM43-E10) purification. The scFv-TM43-E10 antibodies were eluted with extraction buffer containing 250 mM imidazol. The scFv-Fc-TM43-E10 protein was eluted with 100 mM sodium citrate buffer, pH 3.0. Purified scFv-TM43-E10 and scFv-Fc-TM43-E10 antibody fragments were further analysed by sizeexclusion chromatography (SEC) on Superdex 200-16/60 column (GE Healthcare). Calibration was done with Cytochrom C (12.4 kDa), Carbonic Anhydrase (29 kDa), Albumin (66 kDa) and Alcohol Dehydrogenase (150 kDa). The eluted fractions after SEC were investigated by reduced SDS-PAGE and Coomassie blue staining.

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