Author: Schrom, Eva; Huber, Maja; Aneja, Manish; Dohmen, Christian; Emrich, Daniela; Geiger, Johannes; Hasenpusch, Günther; Herrmann-Janson, Annika; Kretzschmann, Verena; Mykhailyk, Olga; Pasewald, Tamara; Oak, Prajakta; Hilgendorff, Anne; Wohlleber, Dirk; Hoymann, Heinz-Gerd; Schaudien, Dirk; Plank, Christian; Rudolph, Carsten; Kubisch-Dohmen, Rebekka
Title: Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Document date: 2017_4_13
ID: tulmnb32_24
Snippet: The flushed lungs were then excised, diced into 2-to 3-mm pieces, and digested in media with collagenase solution, which contained DMEM/F-12, L-glutamine, and 15 mM HEPES (Gibco/Life Technologies) supplemented with 26.5 U/mL collagenase type I (Biochrome). The digested tissue was filtered, washed with PBS, and seeded in culture medium, which contained DMEM/F-12, L-glutamine, 15 mM HEPES, and 10% heat-inactivated FCS (Gibco/Life Technologies), and.....
Document: The flushed lungs were then excised, diced into 2-to 3-mm pieces, and digested in media with collagenase solution, which contained DMEM/F-12, L-glutamine, and 15 mM HEPES (Gibco/Life Technologies) supplemented with 26.5 U/mL collagenase type I (Biochrome). The digested tissue was filtered, washed with PBS, and seeded in culture medium, which contained DMEM/F-12, L-glutamine, 15 mM HEPES, and 10% heat-inactivated FCS (Gibco/Life Technologies), and 1% penicillin/streptomycin (Gibco/Life Technologies). Experiments were performed between passages 4 and 6.
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