Selected article for: "bright field and negative control"
Author: Schrom, Eva; Huber, Maja; Aneja, Manish; Dohmen, Christian; Emrich, Daniela; Geiger, Johannes; Hasenpusch, Günther; Herrmann-Janson, Annika; Kretzschmann, Verena; Mykhailyk, Olga; Pasewald, Tamara; Oak, Prajakta; Hilgendorff, Anne; Wohlleber, Dirk; Hoymann, Heinz-Gerd; Schaudien, Dirk; Plank, Christian; Rudolph, Carsten; Kubisch-Dohmen, Rebekka
Title: Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA
  • Document date: 2017_4_13
    • ID: tulmnb32_50
    Snippet: In situ hybridization of liver tissues was performed by Advanced Cell Diagnostics (ACD), while lung tissues were analyzed by ITEM using a brown RNAscope 2.5 HD Reagent Kit (catalog no. 322300; ACD) following the manufacturer's instructions. For detection of ACE2 cmRNA, a targeted probe was designed by ACD based on the cmRNA sequence provided, while a probe detecting murine ACE2 was derived from GenBank (Genbank: NM_027286.4) RNAscope dapB (bacter.....
    Document: In situ hybridization of liver tissues was performed by Advanced Cell Diagnostics (ACD), while lung tissues were analyzed by ITEM using a brown RNAscope 2.5 HD Reagent Kit (catalog no. 322300; ACD) following the manufacturer's instructions. For detection of ACE2 cmRNA, a targeted probe was designed by ACD based on the cmRNA sequence provided, while a probe detecting murine ACE2 was derived from GenBank (Genbank: NM_027286.4) RNAscope dapB (bacterial dapB; ACD) was used as a negative control, and RNAscope PPIB (cyclophilin B; ACD) as a positive control. Samples were counterstained with hematoxylin and viewed under a bright-field microscope.

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