Author: Mack, Ethan A.; Kallal, Lara E.; Demers, Delia A.; Biron, Christine A.
Title: Type 1 Interferon Induction of Natural Killer Cell Gamma Interferon Production for Defense during Lymphocytic Choriomeningitis Virus Infection Document date: 2011_8_9
ID: qkwo747o_26
Snippet: Adoptive transfers. Total donor PECs from uninfected mice (CD45.2) were isolated as described above and resuspended in 300 l of RPMI medium. These cells were injected i.p. into uninfected recipient mice (CD45.1). Recipient mice were rested for 1 h and either left uninfected or infected with LCMV as described above. Staining to identify donor and recipient cell populations was performed as described below using an anti-CD45.1-fluorescein isothiocy.....
Document: Adoptive transfers. Total donor PECs from uninfected mice (CD45.2) were isolated as described above and resuspended in 300 l of RPMI medium. These cells were injected i.p. into uninfected recipient mice (CD45.1). Recipient mice were rested for 1 h and either left uninfected or infected with LCMV as described above. Staining to identify donor and recipient cell populations was performed as described below using an anti-CD45.1-fluorescein isothiocyanate (FITC) antibody.
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