Author: Evans, Claire F.; Horwitz, Marc S.; Hobbs, Monte V.; Oldstone, Michael B.A.
Title: Viral Infection of Transgenic Mice Expressing a Viral Protein in Oligodendrocytes Leads to Chronic Central Nervous System Autoimmune Disease Document date: 1996_12_1
ID: t82a9y5s_13
Snippet: Generation of Transgenic Mice. The cDNAs of the LCMV NP and GP genes were cloned behind the MBP promoter to direct expression to CNS oligodendrocytes as indicated in Fig. 1 A. This MBP promoter vector had previously been shown to drive expression of a transgene specifically in CNS oligodendrocytes in transgenic mice (18) . Eight founders were identified carrying the LCMV-NP construct, and seven MBP-GP carriers were identified. Because greater tha.....
Document: Generation of Transgenic Mice. The cDNAs of the LCMV NP and GP genes were cloned behind the MBP promoter to direct expression to CNS oligodendrocytes as indicated in Fig. 1 A. This MBP promoter vector had previously been shown to drive expression of a transgene specifically in CNS oligodendrocytes in transgenic mice (18) . Eight founders were identified carrying the LCMV-NP construct, and seven MBP-GP carriers were identified. Because greater than 97% of the LCMV-specific CTL response on the H-2 d background is to an epitope in NP (16), the MBP-NP mice were bred to BALB/cByJ (H-2 d ) mice to maximize the proportion of NP-reactive CTL after LCMV infection. Likewise, because the majority of LCMVspecific CTL react with two epitopes in GP on the H-2 b background (25) , the MBP-GP mice were bred to C57BL/6 (H-2 b ) mice.
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