Coronavirus Infection and PARP Expression Dysregulate the NAD Metabolome: A Potentially Actionable Component of Innate Immunity

Collin D. Heer; Daniel J. Sanderson; Yousef M.O. Alhammad; Mark S. Schmidt; Samuel A.J. Trammell; Stanley Perlman; Michael S. Cohen; Anthony R. Fehr; Charles Brenner

Ferret and human data were obtained from data set GSE147507 deposited by Benjamin tenOever. Data were analyzed using DESeq2 to calculate fold change and p-values (Supplementary Material 4 and 5). Ten genes did not have ferret orthologs in Ensembl and one gene exhibited undetectable expression (TDO2) leaving 60 ferret genes for analysis. Three genes were not present in the human dataset, leaving 68 human genes for analysis. Genes with p > 0.05 (-log(p) > 1.30) were considered statistically significant. Graphs were generated using GraphPad Prism v8. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.17.047480 doi: bioRxiv preprint streptomycin. DBT cells were maintained in serum-free media throughout the infection.