Selected article for: "affinity purification mass spectrometry analysis and purification mass"
Author: Courtney R. Sullivan; Catharine A. Mielnik; Sinead M. O’Donovan; Adam J. Funk; Eduard Bentea; Erica A.K. DePasquale; Zhexing Wen; Vahram Haroutunian; Pavel Katsel; Amy J. Ramsey; Jarek Meller; Robert E. McCullumsmith
Title: Connectivity analyses of bioenergetic changes in schizophrenia: Identification of novel treatments
  • Document date: 2018_6_5
    • ID: ltb6l5xz_110
    Snippet: We used a mouse anti-PSD-95 antibody (Millipore, catalogue # MAB1596) to capture PSD-95 protein complexes from samples (3 male WT, 3 male GluN1 knockdown). We verified the specificity of this antibody using multiple reaction monitoring mass spectrometry analysis of PSD-95 peptides captured by affinity purification. 5ug of PSD-95 antibody was coupled per 1mg of Dynabeads (Life Technologies) according to the antibody coupling kit protocol (#14311D).....
    Document: We used a mouse anti-PSD-95 antibody (Millipore, catalogue # MAB1596) to capture PSD-95 protein complexes from samples (3 male WT, 3 male GluN1 knockdown). We verified the specificity of this antibody using multiple reaction monitoring mass spectrometry analysis of PSD-95 peptides captured by affinity purification. 5ug of PSD-95 antibody was coupled per 1mg of Dynabeads (Life Technologies) according to the antibody coupling kit protocol (#14311D).

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