Selected article for: "ectopic expression and envelope protein"
Author: Keng, Choong-Tat; Åkerström, Sara; Leung, Cynthia Sau-Wai; Poon, Leo L.M.; Peiris, J.S. Malik; Mirazimi, Ali; Tan, Yee-Joo
Title: SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
  • Cord-id: 2yyhnaz7
  • Document date: 2010_10_28
    • ID: 2yyhnaz7
    Snippet: The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed t
    Document: The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.

    Search related documents:
    Co phrase search for related documents
    • accession number and low expression: 1
    • accessory protein and activating enzyme: 1
    • accessory protein and acute sars cov respiratory syndrome coronavirus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
    • accessory protein and low expression: 1, 2
    • acetic acid and lysis buffer: 1, 2
    • actin monoclonal and lysis buffer: 1, 2, 3, 4, 5
    • actin monoclonal antibody and lysis buffer: 1, 2, 3
    • activating enzyme and acute sars cov respiratory syndrome coronavirus: 1
    • activating enzyme and loading buffer: 1
    • acute sars cov respiratory syndrome coronavirus and low expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33
    • acute sars cov respiratory syndrome coronavirus and lysine residue: 1, 2, 3, 4, 5
    • acute sars cov respiratory syndrome coronavirus and lysis buffer: 1, 2, 3
    • loading buffer and lysis buffer: 1, 2, 3, 4, 5, 6