Selected article for: "protein expression and virus production"
Author: Keng, Choong-Tat; Åkerström, Sara; Leung, Cynthia Sau-Wai; Poon, Leo L.M.; Peiris, J.S. Malik; Mirazimi, Ali; Tan, Yee-Joo
Title: SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
  • Cord-id: 2yyhnaz7
  • Document date: 2010_10_28
    • ID: 2yyhnaz7
    Snippet: The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed t
    Document: The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.

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