Author: Moghadasi, Seyad Arad; Becker, Jordan T.; Belica, Christopher; Wick, Chloe; Brown, William L.; Harris, Reuben S.
Title: Gain-of-function assay for SARS-CoV-2 M(pro) inhibition in living cells Cord-id: 22bixx4x Document date: 2020_11_9
ID: 22bixx4x
Snippet: The main protease, M(pro), of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M(pro) function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.
Document: The main protease, M(pro), of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M(pro) function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.
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