Author: A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler
Title: Correlation of mRNA delivery timing and protein expression in lipid-based transfection Document date: 2019_4_13
ID: 9h6ctbyx_27
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/607986 doi: bioRxiv preprint Using Cy5 labeled mRNAs, we also observed that Cy5 fluorescence increased after incubation for about 1 hour. We believe that fluorescence of the Cy5 labeled mRNAs is unquenched during the time of uptake due to unpacking of the lipid-based carriers. Other studies also used multiple fluorescent signals in single cell ti.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/607986 doi: bioRxiv preprint Using Cy5 labeled mRNAs, we also observed that Cy5 fluorescence increased after incubation for about 1 hour. We believe that fluorescence of the Cy5 labeled mRNAs is unquenched during the time of uptake due to unpacking of the lipid-based carriers. Other studies also used multiple fluorescent signals in single cell time lapse measurement to exploit signal correlations (36, 37) . Our findings are in agreement with a study by Yasar et al. showing that the kinetics of mRNA nanocarrier binding on the cell surface is the same for transfected and nontransfected cells without giving further insights on transfection efficiency (26) . In future work, using fluorescent markers, which label endosomes or lysosomes, might reveal intermediate delivery steps and enable further correlation studies with endocytolytic release events.
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