Author: A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler
Title: Correlation of mRNA delivery timing and protein expression in lipid-based transfection Document date: 2019_4_13
ID: 9h6ctbyx_42
Snippet: We determined the delivery onset times from the fluorescence intensity data using a non-linear least-squares fit of the translation-maturation model. We used the three-stage reaction model called translation-maturation model. The model considers a translation step to produce eGFP from mRNA with rate kTL while both mRNA and the protein have decay rates δ and β respectively. The translation step is followed by eGFP maturation, including folding, .....
Document: We determined the delivery onset times from the fluorescence intensity data using a non-linear least-squares fit of the translation-maturation model. We used the three-stage reaction model called translation-maturation model. The model considers a translation step to produce eGFP from mRNA with rate kTL while both mRNA and the protein have decay rates δ and β respectively. The translation step is followed by eGFP maturation, including folding, at rate kM before becoming fluorescent. In the three-stage model we also assume that unmaturated and maturated eGFP decay with the same decay constant as the same proteases are reported for both eGFP states (42) .
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