Author: A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler
Title: Correlation of mRNA delivery timing and protein expression in lipid-based transfection Document date: 2019_4_13
ID: 9h6ctbyx_16
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/607986 doi: bioRxiv preprint Figure 4A ). The mean onset time of 1.6 h for 0% FBS increases to 2.9 h for 10% FBS co-occurring with an increase of the distribution widths revealing a delayed mRNA release into the cytosol with an increased cell-to-cell variability under the presence of serum proteins (see Supplementary Figure 3A ). The expression r.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/607986 doi: bioRxiv preprint Figure 4A ). The mean onset time of 1.6 h for 0% FBS increases to 2.9 h for 10% FBS co-occurring with an increase of the distribution widths revealing a delayed mRNA release into the cytosol with an increased cell-to-cell variability under the presence of serum proteins (see Supplementary Figure 3A ). The expression rate decreases by a factor of four between cells treated with 0% FBS and cells treated with serum, although we did not observe significant differences for the five datasets of cells treated with FBS (see Figure 4A ). We expect that the decrease of the expression rate mainly reflects a decreased number of transfected mRNA molecules m0 and that the translation rate kTL is not affected.
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