Author: A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler
Title: Correlation of mRNA delivery timing and protein expression in lipid-based transfection Document date: 2019_4_13
ID: 9h6ctbyx_46
Snippet: For fitting the fluorescence time courses, we first estimated the starting values for the leastsquares fit. The starting value for the onset time 0 is estimated by determining the time for which the fluorescence intensity has reached 10% of the total intensity range of the time series. Empirically, the fits converge best when a starting value for 0 is chosen that is later than the expected fit result. The starting value for z is set to the median.....
Document: For fitting the fluorescence time courses, we first estimated the starting values for the leastsquares fit. The starting value for the onset time 0 is estimated by determining the time for which the fluorescence intensity has reached 10% of the total intensity range of the time series. Empirically, the fits converge best when a starting value for 0 is chosen that is later than the expected fit result. The starting value for z is set to the median of the first ten data points in the time course. Since the measurement started before transfection, the first data points do not contain protein fluorescence but only background. The starting values for the expression rate and the mRNA degradation rate were set to 0 TL =1000 and =0.02, respectively. The maturation rate and the protein degradation rate were kept fixed to =1.206707417 and =0.004110525, respectively, during fitting.
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