Correlation of mRNA delivery timing and protein expression in lipid-based transfection

A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/607986 doi: bioRxiv preprint shown for 0%, 2%, and 10% FBS in Figure 4B , are uncorrelated as indicated by low Pearson's correlation coefficients r suggesting that there is no intrinsic mechanism connecting fast mRNA delivery with high protein expression rates within a single cell. On the other hand, both parameters show changes with increasing FBS concentrations (see Figure 4C ). While the onset time increases with increasing serum, the efficiency decreases. Hence, the mean expression rates are negatively correlated with the mean onset times (last plot in Figure 4C ) under the variation of the serum concentration. are not correlated at the single-cell level. This is exemplarily shown for the datasets of 0%, 2%, and 10% FBS fraction. Each data point corresponds to a single cell and the Pearson's correlation coefficient r is inscribed. (C) The mean onset times (± standard error of the mean) and the mean expression rate m0kTL are plotted as a function of increasing FBS content. Here, the plot expression rates versus onset times show an inverse dependence (The green data points correspond to the mean value of the six datasets with the color intensity indicating the FBS content, the gray line is to guide the eye). author/funder. All rights reserved. No reuse allowed without permission.