Author: Fan, Zheng; Tan, Xin-yu; Yin, Bin; Zou, Ke; Wang, Ting; Shen, Yan; Ni, An-ping; Qin, Chuan; Yuan, Jian-gang; Qiang, Bo-qin; Peng, Xiao-zhong
Title: [cDNAs cloning of SARS-CoV PUMC2 viral genome]. Cord-id: 59jaz69c Document date: 2003_1_1
ID: 59jaz69c
Snippet: OBJECTIVE To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain. METHODS Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced. RESULTS The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained. CONCLUSIONS These cDNAs can be provided for the function study of SARS-CoV proteins an
Document: OBJECTIVE To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain. METHODS Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced. RESULTS The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained. CONCLUSIONS These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.
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