Author: A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler
Title: Correlation of mRNA delivery timing and protein expression in lipid-based transfection Document date: 2019_4_13
ID: 9h6ctbyx_13
Snippet: Next, we study the influence of nonspecific protein adsorption on the nanocarrier's surface on the onset times and expression levels. Specifically, we increased fetal bovine serum (FBS) fractions within the medium during mRNA lipoplex incubation. To quantify if the transfection ability is systematically dependent on increasing FBS fraction we performed our assay by transfecting the cells using Lipofectamine2000 self-assembled complexes in cell cu.....
Document: Next, we study the influence of nonspecific protein adsorption on the nanocarrier's surface on the onset times and expression levels. Specifically, we increased fetal bovine serum (FBS) fractions within the medium during mRNA lipoplex incubation. To quantify if the transfection ability is systematically dependent on increasing FBS fraction we performed our assay by transfecting the cells using Lipofectamine2000 self-assembled complexes in cell culture medium with six different FBS fractions ranging from 0% to 10 % (v/v) FBS.
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