Correlation of mRNA delivery timing and protein expression in lipid-based transfection

A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/607986 doi: bioRxiv preprint times including the short time scales not accessible otherwise due to need for off-microscope sample handling. In our approach, we used the expression rate as a measure for transfection efficiency. We showed that the expression rate constant m0kTL, the product of released mRNA molecules and the kinetic translation rate, is proportional to the protein level at a defined time point after transfection, which is more frequently used as the measure for transfection efficiency (25, 26) . The proportionality can be explained because the expression rate corresponds to the slope of a straight line between the expression onset and a certain protein level i.e. 12 h after the transfection (see Supplementary Figure 6 ).