Changes in Serum Thiol-Disulphide Homeostasis in Sheep with Gastrointestinal Nematodes

Schmidt, Elizabeth Moreira dos Santos; Fachiolli, Daniele Floriano; de Oliveira, Raphaela Moreira; Almeida, Fabiana Alves; Pariz, Cristiano Magalhães; de Lima Meirelles, Paulo Roberto; Costa, Ciniro; Tvarijonaviciute, Asta; Erel, Ozcan; Neselioglu, Salim; Ceron, Jose Joaquin; Rubio, Camila Peres

SIMPLE SUMMARY: Parasitism with gastrointestinal nematodes represents a significant risk to the health of livestock populations. Besides the local oxidative damage caused by the parasite, the host reacts by increasing the production of oxidants. The study of thiol-disulphide homeostasis can be of help in the evaluation of the oxidative status of sheep during this type of parasitism. In this study, the thiol-disulphide homeostasis, together with other biomarkers of oxidative stress and inflammation, were assessed in the serum of lambs infected with gastrointestinal nematodes and were evaluated after 70 days of integrated crop-livestock system and anthelmintic treatment. This study showed that the thiol-disulphide balance was impaired in the infected lambs and the changes were correlated with the parasite load, which therefore could indicate their potential use as a tool to evaluate and monitor the disease in sheep. ABSTRACT: This work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease.