Author: Jorgensen, Marda; Joseph, Paul; Posgai, Amanda L.; Vander Heide, Richard S.; Kusmartseva, Irina; Atkinson, Mark A.
Title: ACE2 Chromogenic Immunostaining Protocol Optimized for Formalin-fixed Paraffin Embedded Human Tissue Sections Cord-id: 27lgp2u0 Document date: 2021_7_21
ID: 27lgp2u0
Snippet: Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019 (COVID-19). We performed extensive testing of multiple primary antibodies across various human tissue types. Here we describe an optimized protocol for immunostaining of ACE2 in formalin fixed paraffin embedded human (FFPE) pancreas, sma
Document: Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019 (COVID-19). We performed extensive testing of multiple primary antibodies across various human tissue types. Here we describe an optimized protocol for immunostaining of ACE2 in formalin fixed paraffin embedded human (FFPE) pancreas, small intestine and kidney tissue sections obtained from organ donors and autopsies.
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