In silico deceased donor intervention research: A potential accelerant for progress.

Hobeika, Mark J; Casarin, Stefano; Saharia, Ashish; Mobley, Constance; Yi, Stephanie; McMillan, Robert; Ghobrial, R Mark; Gaber, A Osama

Progress in deceased donor intervention research has been limited. Development of an in silico model of deceased donor physiology may elucidate potential therapeutic targets and provide an efficient mechanism for testing proposed deceased donor interventions. In this study we report a preliminary in silico model of deceased kidney donor injury built, calibrated, and validated based on data from published animal and human studies. We demonstrate that the in silico model behaves like animal studies of brain death pathophysiology with respect to upstream markers of renal injury including hemodynamics, oxygenation, cytokines expression, and inflammation. Therapeutic hypothermia, a deceased donor intervention studied in human trials, is performed to demonstrate the model's ability to mimic an established clinical trial. Finally, future directions for developing this concept into a functional, clinically-applicable model are discussed.