Author: A. Reiser; D. Woschée; N. Mehrotra; R. Krzyszton; H. H. Strey; J. O. Rädler
Title: Correlation of mRNA delivery timing and protein expression in lipid-based transfection Document date: 2019_4_13
ID: 9h6ctbyx_6
Snippet: In the first set of experiments Lipofectamine2000 was used as transfection agent and mRNA encoding for eGFP as reporter (see Materials and Methods). The scanning time-lapse measurement sequentially yields fluorescence image stacks from typically 70-100 positions. The movies are further processed to retrieve single-cell protein expression time courses as shown in Figure 1B . Each single-cell time course consists of the mean eGFP fluorescence inten.....
Document: In the first set of experiments Lipofectamine2000 was used as transfection agent and mRNA encoding for eGFP as reporter (see Materials and Methods). The scanning time-lapse measurement sequentially yields fluorescence image stacks from typically 70-100 positions. The movies are further processed to retrieve single-cell protein expression time courses as shown in Figure 1B . Each single-cell time course consists of the mean eGFP fluorescence intensity over the square occupied by a cell during the measurement period resulting in the protein expression kinetic for the respective cell. As expected, the single-cell time courses show a large cell-to-cell variability regarding the eGFP expression (15, 16, 18) . Figure 1C author/funder. All rights reserved. No reuse allowed without permission.
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