A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice

Silva-Cayetano, Alyssa; Foster, William S.; Innocentin, Silvia; Belij-Rammerstorfer, Sandra; Spencer, Alexandra J.; Burton, Oliver T.; Fra-Bidó, Sigrid; Lee, Jia Le; Thakur, Nazia; Conceicao, Carina; Wright, Daniel; Barett, Jordan; Evans-Bailey, Nicola; Noble, Carly; Bailey, Dalan; Liston, Adrian; Gilbert, Sarah C.; Lambe, Teresa; Linterman, Michelle A.

The spread of SARS-CoV-2 has caused a global pandemic that has affected almost every aspect of human life. The development of an effective COVID-19 vaccine could limit the morbidity and mortality caused by infection, and may enable the relaxation of social distancing measures. Age is one of the most significant risk factors for poor health outcomes after SARS-CoV-2 infection, therefore it is desirable that any new vaccine candidates should elicit a robust immune response in older adults. Here, we test the immunogenicity of the adenoviral vectored vaccine ChAdOx1 nCoV-19 (AZD-1222) in aged mice. We find that a single dose of this vaccine induces cellular and humoral immunity in aged mice, but at a reduced magnitude than in younger adult mice. Furthermore, we report that a second dose enhances the immune response to this vaccine in aged mice, indicating that a primeboost strategy may be a rational approach to enhance immunogenicity in older persons.