Design and strategy for spectrofluorimetric determination of Tranexamic acid in its authentic form and pharmaceutical preparations: Application to spiked human plasma.

Anwer, Ebtehal F; Nour El-Deen, Deena A M; Omar, Mahmoud A

A creative, very sensitive and non-complicated spectrofluorimetric technique has been established and further validated to determine Tranexamic acid in both its authentic form and its pharmaceutical preparation dosage forms. In the introduced technique, the reaction is found to be exist between the aliphatic primary amino group of tranexamic acid and ninhydrin/phenylacetaldehyde reagents in the presence of torell and steinhagen buffer its pH is 7.0 which lead to produce a highly fluorescent product and its fluorescence intensity has been measured at 475 nm after excitation at 391 nm. After that, a calibration curve has been drawn with a linear range (0.3-2 μg/ml). Then, LOD and LOQ values has been calculated to be 0.051 and 0.155 μg/ml respectively. The introduced technique has been validated as stated in ICH guidelines and agreed for determination of tranexamic acid in its pharmaceutical formulations. Finally, this simple method was also applied for determination of tranexamic acid in spiked human plasma.