Environmental Detection of SARS-CoV-2 Virus RNA in Health Facilities in Brazil and a Systematic Review on Contamination Sources

Vicente, Vania Aparecida; Lustosa, Bruno Paulo Rodrigues; Grisolia, Maria Eduarda; Pavini Beato, Caroline; Balsanelli, Eduardo; de Souza Gubert Fruet, Viviane; Bordignon Nogueira, Meri; Raboni, Sonia Maria; Carvalho, Katherine Athayde Teixeira; Flôr, Izadora Cervelin; Ferreira Voidaleski, Morgana; Etchepare, Ramiro Gonçalves; Meis, Jacques F.; Soccol, Vanete Thomaz; Souza, Emanuel Maltempi

SARS-CoV-2 environmental monitoring can track the rate of viral contamination and can be used to establish preventive measures. This study aimed to detect by RT-PCR the presence of SARS-CoV-2 from inert surface samples in public health settings with a literature review about surface contamination and its burden on spread virus. Samples were collected from health settings in Curitiba, Brazil, between July and December 2020. A literature review was conducted using PRISMA. A total of 711 environmental surface samples were collected from outpatient areas, dental units, doctors’ offices, COVID-19 evaluation areas, and hospital units, of which 35 (4.9%) were positive for SARS-CoV-2 RNA. The frequency of environmental contamination was higher in primary care units than in hospital settings. The virus was detected on doctors’ personal items. Remarkably, the previously disinfected dental chair samples tested positive. These findings agree with those of other studies in which SARS-CoV-2 was found on inanimate surfaces. Detection of SARS-CoV-2 RNA on surfaces in public health settings, including those not meant to treat COVID-19, indicates widespread environmental contamination. Therefore, the intensification of disinfection measures for external hospital areas may be important for controlling community COVID-19 dissemination.