Author: Brent De Wijngaert; Shemaila Sultana; Chhaya Dharia; Hans Vanbuel; Jiayu Shen; Daniel vasilchuk; Sergio E Martinez; Eaazhisai Kandiah; Smita S Patel; Kalyan Das
Title: Cryo-EM structures reveal transcription initiation steps by yeast mitochondrial RNA polymerase Document date: 2020_4_14
ID: ex3zlq38_2
Snippet: In y-mtPIC structure ( Fig. 1B & C) , the MTF1 is traced from 2 to 336 out of 341 amino acid residues, RPO41 is traced from 386 to the end residue 1351 with few disordered regions, and unambiguously traced DNA (Fig. S3A) . A stable transcription core is composed of RPO41, MTF1, and transcription bubble (Fig. 1C ). RPO41 interacts with MTF1 at multiple locations. Two RPO41 b-hairpins -the intercalating hairpin (ICH) and the MTF1supporting hairpin .....
Document: In y-mtPIC structure ( Fig. 1B & C) , the MTF1 is traced from 2 to 336 out of 341 amino acid residues, RPO41 is traced from 386 to the end residue 1351 with few disordered regions, and unambiguously traced DNA (Fig. S3A) . A stable transcription core is composed of RPO41, MTF1, and transcription bubble (Fig. 1C ). RPO41 interacts with MTF1 at multiple locations. Two RPO41 b-hairpins -the intercalating hairpin (ICH) and the MTF1supporting hairpin (K613-P632) form a crescent-shaped platform that accommodates the C-terminal domain of MTF1 (252-325) (Fig. 1D) . The N-terminal domain of MTF1 contacts the tip of the RPO41 thumb helix (Fig. 1E) ; biochemically, we show the interaction stabilizes RPO41-MTF1 complex ( Fig. S3C -D) (7) . The MTF1-supporting hairpin also guides the MTF C-tail (326-341) towards the active site (Fig. 1F ); the C-tail is disordered in free MTF1(8).
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