Author: Dene Littler; Benjamin Gully; Rhys N Colson; Jamie Rossjohn
Title: Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9 Document date: 2020_3_30
ID: beoseizn_35
Snippet: To examine the RNA-binding affinity, an 18-point serial dilution (212-0 µM) of Nsp9COV19 was incubated with 1 nM 5'-Fluorescein labelled 17mer poly-U single-stranded RNA (Dharmacon GE, USA) or 10mer PolyT single-stranded DNA (IDT, USA) in assay buffer (20mM HEPES pH 7.0, 150mM NaCl, 2mM MgCl2) at room temperature......
Document: To examine the RNA-binding affinity, an 18-point serial dilution (212-0 µM) of Nsp9COV19 was incubated with 1 nM 5'-Fluorescein labelled 17mer poly-U single-stranded RNA (Dharmacon GE, USA) or 10mer PolyT single-stranded DNA (IDT, USA) in assay buffer (20mM HEPES pH 7.0, 150mM NaCl, 2mM MgCl2) at room temperature.
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