Critical Nodes of Virus–Host Interaction Revealed Through an Integrated Network Analysis

Korbinian Bösl; Aleksandr Ianevski; Thoa T. Than; Petter I. Andersen; Suvi Kuivanen; Mona Teppor; Eva Zusinaite; Uga Dumpis; Astra Vitkauskiene; Rebecca J. Cox; Hannimari Kallio-Kokko; Anders Bergqvist; Tanel Tenson; Valentyn Oksenych; Magnar Bjørås; Marit W. Anthonsen; David Shum; Mari Kaarbø; Olli Vapalahti; Marc P. Windisch; Giulio Superti-Furga; Berend Snijder; Denis Kainov; Richard K. Kandasamy

We performed gene-set enrichment analysis using DAVID Bioinformatics Resources (version 6.8, Huang da et al. 2009 ). For all enrichment analysis, a p-value cutoff of ≤0.01 was used as significant. Protein disorder analysis was performed using IUPred2A software. We used the offline version with protein sequences downloaded from UniProt. Statistical analysis of disordered region distribution was performed by Kolmogorov-Smirnov test in R statistical environment. Annotation of human proteins was mapped from UniProt ID to ENSEMBL using EnsDb.Hsapiens.v86. The index of subcellular localization of interaction partners of single viral proteins was calculated for all viral proteins with ≥ five host targets. Localization of host targets was mapped using COMPARTMENTS (Binder et al. 2014) , filtered for a minimum evidence score of 3 in the knowledge channel, excluding non-experimental based preprint author/funder. All rights reserved. No reuse allowed without permission.