Dark Proteome of Newly Emerged SARS-CoV-2 in Comparison with Human and Bat Coronaviruses

Rajanish Giri; Taniya Bhardwaj; Meenakshi Shegane; Bhuvaneshwari R. Gehi; Prateek Kumar; Kundlik Gadhave

The newly emerged SARS-CoV-2 has an ORF10 protein of 38 amino acids. ORF10 of SARS-CoV-2 has a 100% sequence similarity with ORF10 of Bat CoV strain Bat-SL-CoVZC45 [21] . However, we did not conduct the disorder analysis for ORF10 from the Bat-SL-CoVZC45 strain, since all our studies reported here are related to a different strain of Bat CoV (reviewed strain HKU3-1). Therefore, we report here only the results of disorder analysis for the ORF10 protein from SARS-CoV-2, according to which this protein has a mean PPID of 0.00% (see also Figure 15 for disorder profile of ORF10). This protein contains a MoRF from 3-7 residues at its N-terminus as predicted by MoRFchibi_web. Further, we found its tendency to nucleotides and found the presence of few RNA binding sites, however, it does not contain DNA binding residues. Protein ORF14. This is a 70-amino-acid-long uncharacterized protein of unknown function, which is present in Human SARS and Bat CoV. In SARS-CoV-2, ORF14 is a 73-amino-acidlong protein. According to the MSA, ORF14 of SARS-CoV-2 has 77.1% identity with Human-SARS and 72.9% identity with Bat CoV as represented in Figure 16D . We have performed the intrinsic disorder analysis to see the peculiarities of the distribution of disorder predisposition in this protein. Figures 16A, 16B, and 16C show the resulting disorder author/funder. All rights reserved. No reuse allowed without permission.