Selected article for: "binding model and dissociation constant"
Author: Dene Littler; Benjamin Gully; Rhys N Colson; Jamie Rossjohn
Title: Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9
  • Document date: 2020_3_30
    • ID: beoseizn_36
    Snippet: The assay was performed in 96-well non-binding black plates (Greiner Bio-One), with fluorescence anisotropy measured in triplicate using the PHERAstar FS (BMG) with FP 488-520-520 nm filters. The data was corrected using the anisotropy of RNA sample alone, then fitted by a one-site binding model using the Equation, A = (Amax [L])/(KD+[L]), where A is the corrected fluorescence anisotropy; Amax is maximum binding fluorescence anisotropy signal, [L.....
    Document: The assay was performed in 96-well non-binding black plates (Greiner Bio-One), with fluorescence anisotropy measured in triplicate using the PHERAstar FS (BMG) with FP 488-520-520 nm filters. The data was corrected using the anisotropy of RNA sample alone, then fitted by a one-site binding model using the Equation, A = (Amax [L])/(KD+[L]), where A is the corrected fluorescence anisotropy; Amax is maximum binding fluorescence anisotropy signal, [L] is the Nsp9COV19 concentration, and KD is the dissociation equilibrium constant. Amax and KD were used as fitting parameters and nonlinear regression was performed using GraphPad Prism. Measurements were taken after 60 minutes incubation between protein and RNA. Rp.i.m = Shkl [1/(N-1)] 1/2 Si | Ihkl, i - | / Shkl 2 Rfactor = ( S | |Fo| -|Fc| | ) / ( S |Fo| ) -for all data except as indicated in footnote 3. 3 5% of data was used for the Rfree calculation Values in parentheses refer to the highest resolution bin . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.28.013920 doi: bioRxiv preprint

    Search related documents:
    Co phrase search for related documents
    • binding fluorescence and dissociation equilibrium: 1
    • binding fluorescence and high resolution: 1, 2
    • binding model and dissociation equilibrium: 1, 2
    • binding model and equation binding model: 1
    • binding model and GraphPad Prism: 1
    • binding model and high resolution: 1
    • black plate and GraphPad Prism: 1
    • dissociation equilibrium and GraphPad Prism: 1
    • dissociation equilibrium and high resolution: 1, 2
    • fitting parameter and GraphPad Prism: 1