Dark Proteome of Newly Emerged SARS-CoV-2 in Comparison with Human and Bat Coronaviruses

Rajanish Giri; Taniya Bhardwaj; Meenakshi Shegane; Bhuvaneshwari R. Gehi; Prateek Kumar; Kundlik Gadhave

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.13.990598 doi: bioRxiv preprint intrinsic disorder predisposition [74] , N protein is highly disordered in all three SARS viruses analysed in this study ( Table 1) . Graphs in Figures 6C, 6D , and 6E depict the disorder profiles of SARS-CoV-2, Human SARS CoV, and Bat CoV nucleocapsid proteins and show that their N-and C-terminal regions are completely disordered, and all three proteins also contain the central unstructured segment. As expected, the intrinsic disorder predisposition of the N protein of SARS-CoV-2 is remarkably similar to that for the N protein of Human SARS CoV as reported in a previous study [74] . This is further supported by Figure 6F , where PONDR ® VSL2-generated disorder profiles of these three proteins are overlapped to show almost complete coincidence of their major disorder-related features. It is clear that in N-proteins, the N-and C-termini and a log central segment are completely disordered. Figure 6C shows that in the N protein from SARS-CoV-2, residues 1-57, 64-102, 145-162, 166-289, and 362-422 are found to be disordered. Many of these residues are lying within the NTD and CTD regions, and which, due to their structural plasticity, were not crystallized in Human SARS CoV N protein. SARS-CoV-2 has a disordered segment from 168-289 residues while Human SARS has predicted to have an unstructured segment from 145-289 residues. Overall, all three N proteins are found to be highly disordered.