Selected article for: "S1 protein and SARS CoV S1 protein"
Author: Asif Shajahan; Nitin T. Supekar; Anne S. Gleinich; Parastoo Azadi
Title: Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2
  • Document date: 2020_4_3
    • ID: f0xsisdg_44
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 Legends to figures Figure 1 : The SARS-Cov-2 spike proteins recombinatly expressed on HEK293 supernatant were fractionated through SDA-PAGE, subsequently digested by proteases anc analyzed by nLC-NSI-MS/MS. The expression of SARS-Cov-2 spike protein subunits S1 and S2 on HEK 293 culture supernatant showed higher molecular wei.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 Legends to figures Figure 1 : The SARS-Cov-2 spike proteins recombinatly expressed on HEK293 supernatant were fractionated through SDA-PAGE, subsequently digested by proteases anc analyzed by nLC-NSI-MS/MS. The expression of SARS-Cov-2 spike protein subunits S1 and S2 on HEK 293 culture supernatant showed higher molecular weight upon SDS-PAGE than expected, because of glycosylation. Thus, the gel bands corresponding to the molecular weight of 200 kDa to 100 kDa for S1 and 150 kDa to 80 kDa for S2 were cut, proteins were lysed after reduction-alkylation and analyzed by LC-MS/MS (created with biorender.com). representative O-Glycopeptide 320 VQPTESIVR 328 with core 2 type GalNAcGalNeuAc(GlcNAcGalNeuAc) glycan detected on site Thr323 of spike protein subunit 1. author/funder. All rights reserved. No reuse allowed without permission.

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