Dark Proteome of Newly Emerged SARS-CoV-2 in Comparison with Human and Bat Coronaviruses

Rajanish Giri; Taniya Bhardwaj; Meenakshi Shegane; Bhuvaneshwari R. Gehi; Prateek Kumar; Kundlik Gadhave

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.13.990598 doi: bioRxiv preprint 221)) was observed by DISOPRED3 in human SARS and Bat CoV. However, single MoRF (residues 117-132) observed in SARS-CoV-2 by DISOPRED3. MoRFPred also predicts a short MoRF at C-terminus of SARS-CoV-2 (residues 214-222), Human SARS (residues 214-221), and Bat CoV (residues 211-221) ( Table 2, Supplementary Tables 7 and 8) . Furthermore, the M protein from all three viruses displays strong tendency to bind with RNA (as predicted by PPRint and DisoRDPbind) and DNA (as predicted by DRNApred and DisoRDPbind) (see Supplementary Tables 9, 10, and 11). Our understanding on M protein of CoVs (IDPs and MoRF at C-terminus and molecular recognition) elucidates its crucial role in interaction with the N and E proteins for viral assembly.