Dark Proteome of Newly Emerged SARS-CoV-2 in Comparison with Human and Bat Coronaviruses

Rajanish Giri; Taniya Bhardwaj; Meenakshi Shegane; Bhuvaneshwari R. Gehi; Prateek Kumar; Kundlik Gadhave

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.13.990598 doi: bioRxiv preprint Due to the high levels of sequence identity, mean PPIDs of all Nsp7s were found to be identical and equal to 9.64%. Both SARS-CoV-2 and Human SARS Nsp8 proteins were calculated to have a mean PPID of 23.74% and, for Nsp8 of Bat CoV mean disorder is predicted to be 22.22%. Figures 25A, 25B , and 25C display the intrinsic disorder profiles for Nsp7s, whereas Figures 26A, 26B , and 26C represent the predicted intrinsic disorder propensity of Nsp8s. As our analysis suggests, Nsp7s might have a well-predicted structure, while Nsp8s are moderately disordered. Nsp8s are predicted to have a long IDPR (residues in both SARS-CoV-2 and Human SARS, and a bit shorter IDPR in Bat CoV (residues . Furthermore, SARS-CoV Nsp7 using its N-terminus residues (V11, C13, V17, and V21) forms a hydrophobic core with Nsp8 residues (M92, M95, L96, M99, and L103). Additionally, H-bonding takes place between Nsp7 Q24 and Nsp8 T89 residues [143] . These amino acids are the part of MoRFs predicted in Nsp7 and Nsp8 proteins. The results are tabulated in both Table 2 , and Supplementary Tables 7 and 8. Three protein binding regions in Nsp7 of SARS-CoV-2 (residues 1-30, 39-58, and 65-83), Human SARS (residues 1-30, 44-58, and 64-83) , and Bat CoV (residues 1-30, 39-58, and 65-83) was identified by MoRFchibi_web server. Nsp7 was found with the presence of very few nucleotide-binding regions and Nsp8 contains several DNA as well as RNA binding residues (see Supplementary Tables 9, 10, and 11). author/funder. All rights reserved. No reuse allowed without permission.