Author: Devon Chandler-Brown; Anna M. Bueno; Oguzhan Atay; David S. Tsao
Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing Document date: 2020_4_10
ID: eui41zyg_10
Snippet: avoidance of false-positives by examining sequencing data for the correct spike-in or SARS-CoV-2 sequence. To test this hypothesis, we obtained reference materials in which either SARS-CoV-2 (positive control) or human RNA (negative control) is packaged inside of viral particles and suspended in VTM (Seracare). Since polymerase mixes can have varying resiliency to PCR inhibitors, we also evaluated both Luna RT-qPCR and OneTaq RT-PCR kits......
Document: avoidance of false-positives by examining sequencing data for the correct spike-in or SARS-CoV-2 sequence. To test this hypothesis, we obtained reference materials in which either SARS-CoV-2 (positive control) or human RNA (negative control) is packaged inside of viral particles and suspended in VTM (Seracare). Since polymerase mixes can have varying resiliency to PCR inhibitors, we also evaluated both Luna RT-qPCR and OneTaq RT-PCR kits.
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