Author: Waggoner, Jesse J.; Stittleburg, Victoria; Pond, Renee; Saklawi, Youssef; Sahoo, Malaya K.; Babiker, Ahmed; Hussaini, Laila; Kraft, Colleen S.; Pinsky, Benjamin A.; Anderson, Evan J.; Rouphael, Nadine
Title: Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2 Cord-id: 0kqdtuxr Document date: 2020_7_25
ID: 0kqdtuxr
Snippet: Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
Document: Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
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